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Wdfy3tm1a(KOMP)Mbp
Targeted Allele Detail
Nomenclature
Symbol: Wdfy3tm1a(KOMP)Mbp
Name: WD repeat and FYVE domain containing 3; targeted mutation 1a, Mouse Biology Program, UCDavis
MGI ID: MGI:4455365
Synonyms: Wdfy3lacZ
Gene: Wdfy3  Location: Chr5:101832956-102069921 bp, - strand  Genetic Position: Chr5, 48.95 cM
Wdfy3tm1a(KOMP)Mbp/Wdfy3tm1a(KOMP)Mbp mice exhibit severe neurodevelopmental defects

Show the 2 phenotype image(s) involving this allele.

Mutation
origin
Mutant Cell Lines:  DEPD00523_5_A09, DEPD00523_5_A10, DEPD00523_5_A11, DEPD00523_5_A12, DEPD00523_5_B09, DEPD00523_5_B10, DEPD00523_5_B11, DEPD00523_5_C10, DEPD00523_5_C11, DEPD00523_5_C12, DEPD00523_5_D09, DEPD00523_5_D10, DEPD00523_5_D11, DEPD00523_5_D12, DEPD00523_5_E09, DEPD00523_5_E10, DEPD00523_5_E11, DEPD00523_5_F10, DEPD00523_5_F11, DEPD00523_5_F12, DEPD00523_5_G09, DEPD00523_5_G10, DEPD00523_5_G11, DEPD00523_5_H10, DEPD00523_5_H12
Germline Transmission:  Earliest citation of germline transmission: J:225200
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Conditional ready, Hypomorph, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 101953613 of Chromosome 5 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 101952698. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157064)
Generation of the Wdfy3tm1a(KOMP)Mbp and Wdfy3tm1c(KOMP)Mbp alleles
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Expression
In Structures Affected by this Mutation: 4 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 2 lines available
Carrying any Wdfy3 Mutation:  65 strains or lines available
References
Original:  J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010;
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
03/19/2019
MGI 6.13
The Jackson Laboratory