1700020N01Rik^tm1(KOMP)Mbp
Targeted Allele Detail

Summary

• Symbol: 1700020N01Rik^tm1(KOMP)Mbp
• Name: RIKEN cDNA 1700020N01 gene; targeted mutation 1, Mouse Biology Program, UC Davis
• MGI ID: MGI:4454024
• Gene: 1700020N01Rik Location: Chr10:21469044-21498274 bp, + strand Genetic Position: Chr10, 9.79 cM
• IMPC: 1700020N01Rik gene page

Mutation origin

• Mutant Cell Lines: DEPD00512_4_A09, DEPD00512_4_A10, DEPD00512_4_B09, DEPD00512_4_B10, DEPD00512_4_B11, DEPD00512_4_B12, DEPD00512_4_C09, DEPD00512_4_C10, DEPD00512_4_C11, DEPD00512_4_C12, DEPD00512_4_D11, DEPD00512_4_D12, DEPD00512_4_E09, DEPD00512_4_E10, DEPD00512_4_E11, DEPD00512_4_E12, DEPD00512_4_F10, DEPD00512_4_F11, DEPD00512_4_F12, DEPD00512_4_G09, DEPD00512_4_G10, DEPD00512_4_G11, DEPD00512_4_G12, DEPD00512_4_H09, DEPD00512_4_H10, DEPD00512_4_H11, DEPD00512_4_H12
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 412 starting at position 21469228 and ending at position 21469640 of Chromosome 10 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157064)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any 1700020N01Rik Mutation: 13 strains or lines available

References

• Original: J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010
• All: 1 reference(s)