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Targeted Allele Detail
Symbol: Ascl1tm1.1(cre/ERT2)Jejo
Name: achaete-scute family bHLH transcription factor 1; targeted mutation 1.1, Jane Johnson
MGI ID: MGI:4452601
Synonyms: Ascl1-CreER, Ascl1CreERT2
Gene: Ascl1  Location: Chr10:87326681-87329522 bp, - strand  Genetic Position: Chr10, 43.64 cM
Germline Transmission:  Earliest citation of germline transmission: J:171439
Parent Cell Line:  W4 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Allele Type:    Targeted (Inducible, Recombinase)
Inducer:    tamoxifen
Mutations:    Insertion, Intragenic deletion
Mutation detailsA targeting vector was designed to replace the entire coding region of the achaete-scute complex homolog 1 (Ascl1 or Mash1) with a CreERT2 fusion protein and a frt-flanked neomycin (neo) selection cassette. The construct was electroporated into 129S/SvEv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric mice were bred to C57BL/6 mice. These mice were then bred to mice containing Flp-recombinase to remove the Frt-flanked sequence. These mice were then backcrossed at least 5 generations to and outbred ICR strain generate a colony of Ascl1-CreERT2 mice. (J:171439)
Generation of the Ascl1tm1.1(cre/ERT2)Jejo allele
 Tissue activity of this recombinase allele
Driver: Ascl1     Summary of all recombinase alleles driven by Ascl1. Comparative matrix view of recombinase activities

View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Ascl1 Mutation:  23 strains or lines available
Original:  J:171439 Kim EJ, et al., Ascl1 (mash1) defines cells with long-term neurogenic potential in subgranular and subventricular zones in adult mouse brain. PLoS One. 2011;6(3):e18472
All:  44 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.17
The Jackson Laboratory