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Ascl1tm1.1(cre/ERT2)Jejo
Targeted Allele Detail
Nomenclature
Symbol: Ascl1tm1.1(cre/ERT2)Jejo
Name: achaete-scute family bHLH transcription factor 1; targeted mutation 1.1, Jane Johnson
MGI ID: MGI:4452601
Synonyms: Ascl1-CreER, Ascl1CreERT2
Gene: Ascl1  Location: Chr10:87326681-87329522 bp, - strand  Genetic Position: Chr10, 43.64 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:171439
Parent Cell Line:  W4 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Inducible, Recombinase)
Inducer:    tamoxifen
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA targeting vector was designed to replace the entire coding region of the achaete-scute complex homolog 1 (Ascl1 or Mash1) with a CreERT2 fusion protein and a frt-flanked neomycin (neo) selection cassette. The construct was electroporated into 129S/SvEv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric mice were bred to C57BL/6 mice. These mice were then bred to mice containing Flp-recombinase to remove the Frt-flanked sequence. These mice were then backcrossed at least 5 generations to and outbred ICR strain generate a colony of Ascl1-CreERT2 mice. (J:171439)
Generation of the Ascl1tm1.1(cre/ERT2)Jejo allele
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Ascl1     Summary of all recombinase alleles driven by Ascl1. Comparative matrix view of recombinase activities
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Ascl1 Mutation:  23 strains or lines available
References
Original:  J:171439 Kim EJ, et al., Ascl1 (mash1) defines cells with long-term neurogenic potential in subgranular and subventricular zones in adult mouse brain. PLoS One. 2011;6(3):e18472
All:  44 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/23/2021
MGI 6.17
The Jackson Laboratory