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Tmem254atm1e(KOMP)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Tmem254atm1e(KOMP)Wtsi
Name: transmembrane protein 254a; targeted mutation 1e, Wellcome Trust Sanger Institute
MGI ID: MGI:4451424
Gene: Tmem254a  Location: Chr14:25923300-25927672 bp, - strand  Genetic Position: Chr14, 15.19 cM, cytoband B
Mutation
origin
Mutant Cell Lines:  EPD0527_4_A06, EPD0527_4_A08, EPD0527_4_B06, EPD0527_4_C05, EPD0527_4_D05, EPD0527_4_E05, EPD0527_4_G05, EPD0527_4_G07
Germline Transmission:  Unknown
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 25926286 of Chromosome 14 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 2 lines available
Carrying any Tmem254a Mutation:  2 strains or lines available
References
Original:  J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/08/2019
MGI 6.14
The Jackson Laboratory