Tmem254tm1a(KOMP)Wtsi
Targeted Allele Detail
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Symbol: |
Tmem254tm1a(KOMP)Wtsi |
Name: |
transmembrane protein 254; targeted mutation 1a, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:4451359 |
Synonyms: |
Tmem254atm1a(KOMP)Wtsi |
Gene: |
Tmem254 Location: Chr14:25923724-25928096 bp, - strand Genetic Position: Chr14, 15.49 cM
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IMPC: |
Tmem254 gene page |
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Mutant Cell Lines: |
EPD0527_4_A07, EPD0527_4_C07, EPD0527_4_D07, EPD0527_4_E07, EPD0527_4_E08, EPD0527_4_F08, EPD0527_4_G06, EPD0527_4_H05 |
Germline Transmission: |
Unknown
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Parent Cell Line: |
JM8A3.N1 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
KOMP-CSD
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Allele Type: |
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Targeted (Conditional ready, Null/knockout, Reporter) |
Mutation: |
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Insertion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 25924353 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 25926710. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:148605, J:173534)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Tmem254 Mutation: |
2 strains or lines available
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Original: |
J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009; |
All: |
3 reference(s) |
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