Prdm14^{tm1e(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Prdm14^{tm1e(EUCOMM)Wtsi}
• Name: PR domain containing 14; targeted mutation 1e, Wellcome Trust Sanger Institute
• MGI ID: MGI:4441771
• Gene: Prdm14 Location: Chr1:13183681-13197387 bp, - strand Genetic Position: Chr1, 4.1 cM
• Alliance: Prdm14^{tm1e(EUCOMM)Wtsi} page
• IMPC: Prdm14 gene page

Mutation origin

• Mutant Cell Lines: EPD0525_5_B12, EPD0525_5_C10, EPD0525_5_C12, EPD0525_5_E09, EPD0525_5_F09, EPD0525_5_G11, EPD0525_5_G12, EPD0525_5_H11, EPD0525_5_H12
• Germline Transmission: Earliest citation of germline transmission: J:200019
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 13194009 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534, J:200019)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Prdm14 Mutation: 51 strains or lines available

Notes

Cell line EPD0525_5_H11 was successfully used to make chimeric mice. Germline transmission was accomplished. J:200019

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 3 reference(s)