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Asnstm1a(EUCOMM)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Asnstm1a(EUCOMM)Wtsi
Name: asparagine synthetase; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4441742
Synonyms: Asns-
Gene: Asns  Location: Chr6:7675169-7693254 bp, - strand  Genetic Position: Chr6, 3.31 cM, cytoband A1
Mutation
origin
Mutant Cell Lines:  EPD0525_1_A02, EPD0525_1_A03, EPD0525_1_A04, EPD0525_1_B01, EPD0525_1_B02, EPD0525_1_B03, EPD0525_1_C01, EPD0525_1_C02, EPD0525_1_C03, EPD0525_1_D01, EPD0525_1_D02, EPD0525_1_D03, EPD0525_1_E01, EPD0525_1_E02, EPD0525_1_E03, EPD0525_1_E04, EPD0525_1_F01, EPD0525_1_F02, EPD0525_1_F03, EPD0525_1_G01, EPD0525_1_G02, EPD0525_1_G03, EPD0525_1_G04, EPD0525_1_H01, EPD0525_1_H02, EPD0525_1_H03 (Wellcome Trust Sanger Institute)
Germline Transmission:  Earliest citation of germline transmission: J:165965
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Hypomorph, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_gt0
 
Mutation detailsThe L1L2_gt0 cassette was inserted at position 7685826 of Chromosome 6 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 7684884. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other KOMP alleles can be found at http://www.knockoutmouse.org/aboutkompstrategies. (J:155845, J:173534)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Asns Mutation:  23 strains or lines available
Notes
Special note about mutant cell line EPD0525_1_F01: The mutation generated in this ES cell line results in a hypomorphic allele. qRT-PCR analysis of adult brains revealed that homozygous mutant mice express ~20% of the mRNA level expressed in wild-type brains (J:236441).
References
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
03/21/2017
MGI 6.08
The Jackson Laboratory