Golgb1^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Golgb1^{tm1a(EUCOMM)Hmgu}
• Name: golgin B1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4436000
• Gene: Golgb1 Location: Chr16:36695502-36753447 bp, + strand Genetic Position: Chr16, 26.19 cM, cytoband B4
• Alliance: Golgb1^{tm1a(EUCOMM)Hmgu} page
• IMPC: Golgb1 gene page

Mutation origin

• Mutant Cell Lines: HEPD0073_1_A10, HEPD0073_1_A11, HEPD0073_1_B10, HEPD0073_1_B12, HEPD0073_1_C10, HEPD0073_1_C11, HEPD0073_1_D11, HEPD0073_1_E10, HEPD0073_1_E12, HEPD0073_1_F10, HEPD0073_1_F12, HEPD0073_1_G10, HEPD0073_1_G12, HEPD0073_1_H10
• Germline Transmission: Earliest citation of germline transmission: J:235669
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 36718389 of Chromosome 16 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 36719552. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Golgb1 Mutation: 115 strains or lines available

Notes

J:235669 reports that germline transmission of the targeted mutation was not achieved. However, the cell lines used were not reported. The IMPC and the European Mouse Mutant Repository (EMMA) report germline transmission of this allele and the derivative tm1b allele from cell line HEPD0073_1_G12.

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)