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Smug1tm1a(EUCOMM)Hmgu
Targeted Allele Detail
Nomenclature
Symbol: Smug1tm1a(EUCOMM)Hmgu
Name: single-strand selective monofunctional uracil DNA glycosylase; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:4435952
Gene: Smug1  Location: Chr15:103153290-103167092 bp, - strand  Genetic Position: Chr15, 58.2 cM
Mutation
origin
Mutant Cell Lines:  HEPD0529_1_A08, HEPD0529_1_B07, HEPD0529_1_B08, HEPD0529_1_C05, HEPD0529_1_F08
Germline Transmission:  Earliest citation of germline transmission: J:197809
Parent Cell Line:  JM8.N4 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Pgk_P
 
Mutation detailsThe L1L2_Pgk_P cassette was inserted at position 103156638 of Chromosome 15 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 103153056. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 7 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 1 line available
Carrying any Smug1 Mutation:  9 strains or lines available
Notes
Cell line HEPD0529_1_C05 and HEPD0529_1_B07 were successfully used to make chimeric mice. Germline transmission was accomplished. J:197809
References
Original:  J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015;
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
09/05/2019
MGI 6.14
The Jackson Laboratory