Ythdf1^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Ythdf1^{tm1a(EUCOMM)Hmgu}
• Name: YTH N6-methyladenosine RNA binding protein 1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4435875
• Gene: Ythdf1 Location: Chr2:180546170-180562729 bp, - strand Genetic Position: Chr2, 103.5 cM
• Alliance: Ythdf1^{tm1a(EUCOMM)Hmgu} page
• IMPC: Ythdf1 gene page

Mutation origin

• Mutant Cell Lines: HEPD0602_1_B07, HEPD0602_1_D07, HEPD0602_1_E06, HEPD0602_1_E08, HEPD0602_1_F07, HEPD0602_1_H08, HEPD1054_2_A03, HEPD1054_2_A05, HEPD1054_2_A08, HEPD1054_2_A10, HEPD1054_2_B01, HEPD1054_2_B02, HEPD1054_2_B12, HEPD1054_2_C01, HEPD1054_2_C04, HEPD1054_2_C06, HEPD1054_2_C07, HEPD1054_2_C08, HEPD1054_2_C11, HEPD1054_2_C12, HEPD1054_2_D05, HEPD1054_2_D09, HEPD1054_2_E08, HEPD1054_2_F01, HEPD1054_2_F07, HEPD1054_2_G01, HEPD1054_2_G05, HEPD1054_2_G06, HEPD1054_2_G09, HEPD1054_2_G11, HEPD1054_2_G12, HEPD1054_2_H08, HEPD1054_2_H09, HEPD1054_3_A01, HEPD1054_3_A04, HEPD1054_3_A06, HEPD1054_3_A07, HEPD1054_3_B04, HEPD1054_3_B08, HEPD1054_3_B11, HEPD1054_3_C05, HEPD1054_3_C06, HEPD1054_3_D01, HEPD1054_3_D04, HEPD1054_3_D06, HEPD1054_3_D08, HEPD1054_3_D09, HEPD1054_3_D12, HEPD1054_3_E04, HEPD1054_3_E07, HEPD1054_3_E11, HEPD1054_3_F02, HEPD1054_3_F03, HEPD1054_3_F04, HEPD1054_3_F10, HEPD1054_3_F12, HEPD1054_3_G02, HEPD1054_3_G03, HEPD1054_3_G07, HEPD1054_3_G09, HEPD1054_3_G12, HEPD1054_3_H03, HEPD1054_3_H04, HEPD1054_3_H05, HEPD1054_3_H07, HEPD1054_3_H08, HEPD1054_3_H09
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 180552208 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 180554425. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ythdf1 Mutation: 35 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)