Ralgapa2^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Ralgapa2^{tm1a(EUCOMM)Hmgu}
• Name: Ral GTPase activating protein, alpha subunit 2 (catalytic); targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4435335
• Gene: Ralgapa2 Location: Chr2:146081799-146354264 bp, - strand Genetic Position: Chr2, 71.33 cM
• IMPC: Ralgapa2 gene page

Mutation origin

• Mutant Cell Lines: HEPD0074_2_A10, HEPD0074_2_A11, HEPD0074_2_A12, HEPD0074_2_B11, HEPD0074_2_C10, HEPD0074_2_C11, HEPD0074_2_C12, HEPD0074_2_D10, HEPD0074_2_D11, HEPD0074_2_D12, HEPD0074_2_E11, HEPD0074_2_E12, HEPD0074_2_F10, HEPD0074_2_F11, HEPD0074_2_G10, HEPD0074_2_H10, HEPD0074_2_H11
• Germline Transmission: Unknown
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 146321020 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 146321771. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ralgapa2 Mutation: 116 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 1 reference(s)