Marchf8^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Marchf8^{tm1a(EUCOMM)Hmgu}
• Name: membrane associated ring-CH-type finger 8; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4434858
• Gene: Marchf8 Location: Chr6:116314985-116386501 bp, + strand Genetic Position: Chr6, 53.78 cM, cytoband F1
• IMPC: Marchf8 gene page

Mutation origin

• Mutant Cell Lines: HEPD0510_9_A02, HEPD0510_9_A04, HEPD0510_9_B01, HEPD0510_9_B02, HEPD0510_9_B04, HEPD0510_9_C01, HEPD0510_9_C02, HEPD0510_9_C04, HEPD0510_9_D01, HEPD0510_9_D02, HEPD0510_9_D03, HEPD0510_9_E01, HEPD0510_9_E02, HEPD0510_9_E03, HEPD0510_9_E04, HEPD0510_9_F01, HEPD0510_9_F03, HEPD0510_9_F04, HEPD0510_9_G02, HEPD0510_9_G03, HEPD0510_9_G04, HEPD0510_9_H01, HEPD0510_9_H02, HEPD0510_9_H03, HEPD0510_9_H04
• Germline Transmission: Unknown
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 116379939 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 116380799. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Marchf8 Mutation: 39 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 1 reference(s)