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Targeted Allele Detail
Symbol: Prmt5tm2a(EUCOMM)Wtsi
Name: protein arginine N-methyltransferase 5; targeted mutation 2a, Wellcome Trust Sanger Institute
MGI ID: MGI:4432368
Gene: Prmt5  Location: Chr14:54507187-54517525 bp, - strand  Genetic Position: Chr14, 27.82 cM, cytoband C1
Mutant Cell Lines:  EPD0160_2_A07, EPD0160_2_A08, EPD0160_2_B06, EPD0160_2_B08, EPD0160_2_C06, EPD0160_2_C07, EPD0160_2_D06, EPD0160_2_D08, EPD0160_2_F06, EPD0160_2_G05, EPD0160_2_G06, EPD0160_2_H06 (Wellcome Trust Sanger Institute)
Germline Transmission:  Earliest citation of germline transmission: J:149352
Parent Cell Line:  JM8A3 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 54513936 of Chromosome 14 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 54513002. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other KOMP alleles can be found at http://www.knockoutmouse.org/aboutkompstrategies. (J:155845, J:173534, J:226257)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Prmt5 Mutation:  20 strains or lines available
Mice (purchased from EUCOMM) were generated from cell line EPD0160_2_A08 in J:201152
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  7 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
MGI 6.08
The Jackson Laboratory