Kitl^tm2.1Pbes
Targeted Allele Detail

Summary

• Symbol: Kitl^tm2.1Pbes
• Name: kit ligand; targeted mutation 2.1, Peter Besmer
• MGI ID: MGI:4430867
• Synonyms: KitL^L263A
• Gene: Kitl Location: Chr10:99851492-99936278 bp, + strand Genetic Position: Chr10, 51.4 cM
• Alliance: Kitl^tm2.1Pbes page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:157170
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Leucine 263 in exon 9 was changed to alanine by site directed mutagenesis. A neomycin resistance cassette flanked by loxP sites was inserted 0.5 kb downstream of exon 9. Sequencing analysis confirmed the mutation. The floxed neomycin cassette was excised in vivo by Cre mediated recombination. Western blot analysis using extracts from the cerebellum showed that the total protein expression levels were similar to wild-type mice. Immunostaining showed reduced or absent expression along the Sertoli cell basal membrane. (J:157170)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kitl Mutation: 92 strains or lines available

References

• Original: J:157170 Deshpande S, et al., Kit ligand cytoplasmic domain is essential for basolateral sorting in vivo and has roles in spermatogenesis and hematopoiesis. Dev Biol. 2010 Jan 15;337(2):199-210
• All: 1 reference(s)