Tg(Camk2a-cre/ERT2)1Aibs
Transgene Detail
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Symbol: |
Tg(Camk2a-cre/ERT2)1Aibs |
Name: |
transgene insertion 1, Allen Institute for Brain Science |
MGI ID: |
MGI:4421787 |
Synonyms: |
Camk2a-CreERT2 |
Transgene: |
Tg(Camk2a-cre/ERT2)1Aibs Location: unknown
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Alliance: |
Tg(Camk2a-cre/ERT2)1Aibs page
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Transgene Type: |
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Transgenic (Inducible, Recombinase) |
Inducer: |
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tamoxifen |
Mutation: |
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Insertion
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Tg(Camk2a-cre/ERT2)1Aibs expression driven by
1 gene
Transgene expression driven by:
Organism |
Driver Gene |
Note |
mouse |
Camk2a (MGI:88256) |
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Mutation details: The Camk2a-CreERT2 transgene was designed with the mouse Camk2a promoter, CreERT2 fusion gene, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), bovine growth hormone polyA signal, and PGK-Puro-SV40polyA cassette. (129S6/SvEvTac x C57BL/6)F1-derived G4 embryonic stem (ES) cells, already targeted with an Ai3 mutation in the Gt(ROSA)26Sor locus, were transfected with the Camk2a-CreERT2 transgene. Correctly targeted ES cells harboring both the Ai3 mutation and Camk2a-CreERT2 transgene were injected into recipient blastocysts. The resulting chimeric animals were bred to C57BL/6J mice to generate the double mutant colony. Subsequent mating with C57BL/6J wildtype mice resulted in offspring harboring only the Camk2a-CreERT2 transgene (wildtype at the Gt(ROSA)26Sor locus).
(J:155793)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Camk2a
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Original: |
J:155793 Madisen L, et al., A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci. 2010 Jan;13(1):133-40 |
All: |
48 reference(s) |
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