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Tg(Camk2a-cre/ERT2)1Aibs
Transgene Detail
Summary
Symbol: Tg(Camk2a-cre/ERT2)1Aibs
Name: transgene insertion 1, Allen Institute for Brain Science
MGI ID: MGI:4421787
Synonyms: Camk2a-CreERT2
Transgene: Tg(Camk2a-cre/ERT2)1Aibs  Location: unknown  
Alliance: Tg(Camk2a-cre/ERT2)1Aibs page
Transgene
origin
Germline Transmission:  Earliest citation of germline transmission: J:155793
Parent Cell Line:  G4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Transgene
description
Transgene Type:    Transgenic (Inducible, Recombinase)
Inducer:    tamoxifen
Mutation:    Insertion
 
Tg(Camk2a-cre/ERT2)1Aibs expression driven by 1 gene
 
Mutation detailsThe Camk2a-CreERT2 transgene was designed with the mouse Camk2a promoter, CreERT2 fusion gene, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), bovine growth hormone polyA signal, and PGK-Puro-SV40polyA cassette. (129S6/SvEvTac x C57BL/6)F1-derived G4 embryonic stem (ES) cells, already targeted with an Ai3 mutation in the Gt(ROSA)26Sor locus, were transfected with the Camk2a-CreERT2 transgene. Correctly targeted ES cells harboring both the Ai3 mutation and Camk2a-CreERT2 transgene were injected into recipient blastocysts. The resulting chimeric animals were bred to C57BL/6J mice to generate the double mutant colony. Subsequent mating with C57BL/6J wildtype mice resulted in offspring harboring only the Camk2a-CreERT2 transgene (wildtype at the Gt(ROSA)26Sor locus). (J:155793)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Camk2a (mouse)
Summary of all recombinase alleles driven by Camk2a.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:155793 Madisen L, et al., A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci. 2010 Jan;13(1):133-40
All:  48 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory