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Tg(Camk2a-cre/ERT2)1Aibs
Transgene Detail
Nomenclature
Symbol: Tg(Camk2a-cre/ERT2)1Aibs
Name: transgene insertion 1, Allen Institute for Brain Science
MGI ID: MGI:4421787
Synonyms: Camk2a-CreERT2
Transgene: Tg(Camk2a-cre/ERT2)1Aibs  Location: unknown  
Transgene
origin
Germline Transmission:  Earliest citation of germline transmission: J:155793
Parent Cell Line:  G4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Transgene
description
Transgene Type:    Transgenic (Inducible, Recombinase)
Inducer:    tamoxifen
Mutation:    Insertion
 
Mutation detailsThe Camk2a-CreERT2 transgene was designed with the mouse Camk2a promoter, CreERT2 fusion gene, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), bovine growth hormone polyA signal, and PGK-Puro-SV40polyA cassette. (129S6/SvEvTac x C57BL/6)F1-derived G4 embryonic stem (ES) cells, already targeted with an Ai3 mutation in the Gt(ROSA)26Sor locus, were transfected with the Camk2a-CreERT2 transgene. Correctly targeted ES cells harboring both the Ai3 mutation and Camk2a-CreERT2 transgene were injected into recipient blastocysts. The resulting chimeric animals were bred to C57BL/6J mice to generate the double mutant colony. Subsequent mating with C57BL/6J wildtype mice resulted in offspring harboring only the Camk2a-CreERT2 transgene (wildtype at the Gt(ROSA)26Sor locus). (J:155793)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Camk2a     Summary of all recombinase alleles driven by Camk2a. Comparative matrix view of recombinase activities
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:155793 Madisen L, et al., A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci. 2010 Jan;13(1):133-40
All:  20 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/08/2019
MGI 6.14
The Jackson Laboratory