Nhsl3^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Nhsl3^{tm1(KOMP)Wtsi}
• Name: NHS like 3; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4419150
• Gene: Nhsl3 Location: Chr4:129113371-129155194 bp, - strand Genetic Position: Chr4, 63.11 cM
• IMPC: Nhsl3 gene page

Mutation origin

• Mutant Cell Lines: EPD0411_3_A10, EPD0411_3_A11, EPD0411_3_B10, EPD0411_3_B12, EPD0411_3_C09, EPD0411_3_C10, EPD0411_3_C12, EPD0411_3_D10, EPD0411_3_D11, EPD0411_3_D12, EPD0411_3_E09, EPD0411_3_F09, EPD0411_3_F10, EPD0411_3_F12, EPD0411_3_G09, EPD0411_3_G10, EPD0411_3_G11, EPD0411_3_H10
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 4290 starting at position 129115565 and ending at position 129119855 of Chromosome 4 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Nhsl3 Mutation: 113 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 2 reference(s)