F2r^tm1.1Cgh
Targeted Allele Detail

Summary

• Symbol: F2r^tm1.1Cgh
• Name: coagulation factor II thrombin receptor; targeted mutation 1.1, Shaun R Coughlin
• MGI ID: MGI:4365911
• Synonyms: Par1-LacZ
• Gene: F2r Location: Chr13:95738311-95754995 bp, - strand Genetic Position: Chr13, 50.21 cM
• Alliance: F2r^tm1.1Cgh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71309
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The starting ATG codon as well as codons 2-4 of exon 1 were replaced with a lacZ-floxed PGKneo cassette. The neo cassette was in the same transcriptional orientation as the gene itself. In vivo cre excision was accomplished by crossing to Tg(ACTB-cre)2Mrt transgenic mice. After cre excision of neo, beta galactosidase staining was reduced in intensity but the overall pattern of staining was maintained. (J:71309, J:157446)

Expression

In Mice Carrying this Mutation:

15 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any F2r Mutation: 23 strains or lines available

References

• Original: J:71309 Griffin CT, et al., A role for thrombin receptor signaling in endothelial cells during embryonic development. Science. 2001 Aug 31;293(5535):1666-70
• All: 4 reference(s)