Marchf10tm1a(KOMP)Wtsi
Targeted Allele Detail
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Symbol: |
Marchf10tm1a(KOMP)Wtsi |
Name: |
membrane associated ring-CH-type finger 10; targeted mutation 1a, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:4363847 |
Gene: |
Marchf10 Location: Chr11:105251624-105347561 bp, - strand Genetic Position: Chr11, 68.73 cM
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IMPC: |
Marchf10 gene page |
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Mutant Cell Lines: |
EPD0121_5_A05, EPD0121_5_A06, EPD0121_5_D08, EPD0121_5_E05, EPD0121_5_G07 |
Germline Transmission: |
Unknown
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Parent Cell Line: |
JM8.N4 (ES Cell)
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Strain of Origin: |
C57BL/6N
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Project Collection: |
KOMP-CSD
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Allele Type: |
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Targeted (Conditional ready, Null/knockout, Reporter) |
Mutation: |
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Insertion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 105299207 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 105300087. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:148605, J:173534)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Marchf10 Mutation: |
35 strains or lines available
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Original: |
J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009; |
All: |
3 reference(s) |
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