Kdm5b^{Gt(RRO123)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Kdm5b^{Gt(RRO123)Byg}
• Name: lysine demethylase 5B; gene trap RRO123, BayGenomics
• MGI ID: MGI:4329395
• Synonyms: Jarid1b^-
• Gene: Kdm5b Location: Chr1:134487916-134560621 bp, + strand Genetic Position: Chr1, 58.24 cM, cytoband E4
• Alliance: Kdm5b^{Gt(RRO123)Byg} page

Mutation origin

• Mutant Cell Line: RRO123
• Germline Transmission: Earliest citation of germline transmission: J:218960
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT2Lxf Vector Type: gene trap
• Mutation details: A gene trap vector containing a splice-acceptor sequence upstream of beta-geo (a fusion of beta-galactosidase and neomycin phosphotransferase II) was introduced to intron 2 of the gene, resulting in premature termination of the protein before the AT-rich interactive domain (ARID). Western blot analysis using an antibody against the C terminus shows that full-length protein is undetectable. (J:218960)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRO123	CG671974 ; (MGI Seq Detail)	5' RACE	Chr1:134488122-134512966 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  200 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Kdm5b Mutation: 198 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

E14TG2a.4 embryonic stem cells

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)