Tgm3^{Gt(IST12449B1)Tigm}
Gene trapped Allele Detail

Summary

• Symbol: Tgm3^{Gt(IST12449B1)Tigm}
• Name: transglutaminase 3, E polypeptide; gene trap IST12449B1, Texas A&M Institute for Genomic Medicine
• MGI ID: MGI:4077572
• Gene: Tgm3 Location: Chr2:129854269-129892319 bp, + strand Genetic Position: Chr2, 63.19 cM
• Alliance: Tgm3^{Gt(IST12449B1)Tigm} page

Mutation origin

• Mutant Cell Line: IST12449B1
• Germline Transmission: Earliest citation of germline transmission: J:277352
• Parent Cell Line: Lex3.13 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion of gene trap vector Vector: VICTR76 Vector Type: gene trap
• Mutation details: Trapped gene association determined by genome coordinate overlap and strand. The insertion site was identified in intron 1. RT-PCR and Western blot confirmed the absence of RNA and protein. (J:277352)
• Sequence Tags: Sequence tag details (3 tags)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  IST12449B1HMF1	JM198714 ; (MGI Seq Detail)	inverse PCR	Chr2:129854735-129854874 bp (+)
  IST12449B1HMF1	EF924388 ; (MGI Seq Detail)	inverse PCR	Chr2:129854735-129854874 bp (-)
  IST12449B1BBF1	EF966470 ; (MGI Seq Detail)	inverse PCR	Chr2:129854616-129854874 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  1 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Tgm3 Mutation: 53 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 2 reference(s)