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Targeted Allele Detail
Symbol: Myrftm1Barr
Name: myelin regulatory factor; targeted mutation 1, Ben Barres
MGI ID: MGI:3851143
Synonyms: MRFfl, MyrfloxP
Gene: Myrf  Location: Chr19:10208272-10240748 bp, - strand  Genetic Position: Chr19, 6.54 cM
Germline Transmission:  Earliest citation of germline transmission: J:150563
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
Mutation detailsA targeting vector was used to flank exon 8 of the endogenous gene with loxP sites. The vector also contained an FRT-flanked neomycin resistance cassette. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. Heterozygous mice were crossed to the FlpER (129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym) strain to remove the neo cassette, leaving a single FRT site upstream of exon 8 and the flanking loxP sites. (J:150563)
View phenotypes and curated references for all genotypes (concatenated display).
In Mice Carrying this Mutation: 53 assay results
In Structures Affected by this Mutation: 6 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Myrf Mutation:  40 strains or lines available
Original:  J:150563 Emery B, et al., Myelin gene regulatory factor is a critical transcriptional regulator required for CNS myelination. Cell. 2009 Jul 10;138(1):172-85
All:  9 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
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