Tg(CAG-Bgeo,-tTA,-EGFP)2A11Kuw
Transgene Detail

Summary

• Symbol: Tg(CAG-Bgeo,-tTA,-EGFP)2A11Kuw
• Name: transgene insertion 2A11, Kay-Uwe Wagner
• MGI ID: MGI:3849835
• Synonyms: CAG-betageo-tTA, CAG-bGeo-tTA-IRES-GFP, CAG-loxP-betageo-loxP-tTA-IRES-GFP, CAG-tTA-IRES-GFP, pCCALL2-tTA-IRES-GFP
• Transgene: Tg(CAG-Bgeo,-tTA,-EGFP)2A11Kuw Location: unknown
• Alliance: Tg(CAG-Bgeo,-tTA,-EGFP)2A11Kuw page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:153278
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Transgene description

• Transgene Type: Transgenic (Conditional ready, Reporter, Transactivator)
• Mutation: Insertion
• Mutation details: This transgene contains the "CAG promoter" -- consisting of the CMV-IE enhancer followed by the chicken beta-actin (ACTB) upstream promoter, first exon and first intron -- driving expression of a loxP-flanked lacZ/neo^R fusion gene (betageo) with three copies of the SV40 polyadenylation signal (3xSV40p[A]). Upon excision of betageo by Cre recombinase, the tetracycline transactivator (tTA) and enhanced green fluorescent protein (EGFP) genes are expressed from the CAG promoter. Presence of the tTA protein promotes expression of other transgenes regulated by the tet operon (tetO); this expression is readily suppressed by doxycycline. (J:153278)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:153278 Zhang Q, et al., Temporally and spatially controlled expression of transgenes in embryonic and adult tissues. Transgenic Res. 2010 Jun;19(3):499-509
• All: 3 reference(s)