Tg(Foxp3-HBEGF/EGFP)23.2Spar
Transgene Detail

Summary

• Symbol: Tg(Foxp3-HBEGF/EGFP)23.2Spar
• Name: transgene insertion 23.2, Tim Sparwasser
• MGI ID: MGI:3849080
• Synonyms: DEREG, Foxp3-IRES-DTR-GFP BAC-Tg, Tg(Foxp3-DTR/EGFP)1Ebe, Tg(Foxp3-DTR/EGFP)23.2Spar
• Transgene: Tg(Foxp3-HBEGF/EGFP)23.2Spar Location: unknown
• Alliance: Tg(Foxp3-HBEGF/EGFP)23.2Spar page

Transgene origin

• Strain of Origin: C57BL/6NCrl

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Foxp3-HBEGF/EGFP)23.2Spar expresses 2 genes
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  mouse

  Foxp3 (MGI:1891436)

  human

  HBEGF (1839)

  Hbegf

  (MGI:96070)

• Mutation details: The RP23-267C15 bacterial artificial chromosome (BAC) containing at least ten mouse genes was obtained. This BAC was modified by targeting a simian diphtheria toxin receptor-enhanced green fluorescent protein (DTR-eGFP) fusion protein and an SV40 polyA element into the first exon of the Foxp3 (forkhead box P3) locus: none of the other loci on the BAC were mutated. Two high expressing lines (16.1 and 23.2) were established. Analysis revealed selective DTR-eGFP expression within the CD4+ T cell compartment, with the highest expression within the CD25+ subset. (J:125295)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:125295 Lahl K, et al., Selective depletion of Foxp3+ regulatory T cells induces a scurfy-like disease. J Exp Med. 2007 Jan 22;204(1):57-63
• All: 152 reference(s)