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Targeted Allele Detail
Symbol: Ightm3Mnz
Name: immunoglobulin heavy chain complex; targeted mutation 3, Michel C Nussenzweig
MGI ID: MGI:3836312
Synonyms: IgHI
Gene: Igh  Location: unknown  Genetic Position: Chr12, Syntenic
Germline Transmission:  Earliest citation of germline transmission: J:145691
Parent Cell Line:  CY2.4 (ES Cell)
Strain of Origin:  B6(Cg)-Tyrc-2J
Allele Type:    Targeted (Not Applicable)
Mutation:    Insertion
Mutation detailsA targeting vector containing a self-excising loxP-flanked neomycin/sperm-specific Cre cassette was used to target a loxP site and a I-SceI endonuclease site upstream of the S-mu switching region and downstream of the E-mu enhancer. The construct was electroporated into albino C57BL/6 embryonic stem (ES) cells. Correctly targeted ES clones were injected into recipient blastocysts. The resulting male founder had self-excision of the neomycin/Cre cassette, leaving the loxP and I-SceI sites in the germline. Correct targeting was confirmed by Southern blotting. B cell development and numbers were normal in heterozygote mice. (J:145691)
Generation of the Ightm3Mnz allele
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Igh Mutation:  44 strains or lines available
Original:  J:145691 Robbiani DF, et al., AID is required for the chromosomal breaks in c-myc that lead to c-myc/IgH translocations. Cell. 2008 Dec 12;135(6):1028-38
All:  7 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
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