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Gt(ROSA)26Sortm4(Wnt7a)Amc
Targeted Allele Detail
Nomenclature
Symbol: Gt(ROSA)26Sortm4(Wnt7a)Amc
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 4, Andrew P McMahon
MGI ID: MGI:3831175
Synonyms: R26-C-Wnt7aIL, R26-Wnt7aIRESLacZ, ROSA26-CAGGS-Wnt7a
Gene: Gt(ROSA)26Sor  Location: Chr6:113067428-113077244 bp, - strand  Genetic Position: Chr6, 52.73 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:142352
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  Not Specified
Mutation
description
Allele Type:    Targeted (Conditional ready, Inserted expressed sequence, Reporter)
Mutation:    Insertion
 
Gt(ROSA)26Sortm4(Wnt7a)Amc expresses 1 gene
 
Mutation detailsA cassette containing a splice acceptor, the puromycin resistance gene, and a poly A sequence was placed in front of a CAGGS promoter. Downstream of the promoter was placed a floxed terminator element containing three SV40 poly A sequences (tpA) followed by a bicistronic cassette encoding for Wnt7a and IRES-nlacZ. In the absence of Cre recombinase, transcription from the CAGGS enhancer/promoter is terminated by the tpA element. In the presence of cre recombinase, the tpA is recombined out and the CAGGS enhancer/promoter drives expression of both Wnt7a and betagalactosidase. Ectopic Wnt7a and lacZ expression were detected in the neuroepithelium of developing embryos when this allele was on a Tg(Nes-cre)1Kln transgenic background. (J:142352)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  497 strains or lines available
References
Original:  J:142352 Stenman JM, et al., Canonical Wnt signaling regulates organ-specific assembly and differentiation of CNS vasculature. Science. 2008 Nov 21;322(5905):1247-50
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
09/10/2019
MGI 6.14
The Jackson Laboratory