Adar^tm1.1Phs
Targeted Allele Detail

Summary

• Symbol: Adar^tm1.1Phs
• Name: adenosine deaminase, RNA-specific; targeted mutation 1.1, Peter H Seeburg
• MGI ID: MGI:3828307
• Synonyms: Adar^f, adar^f7-9
• Gene: Adar Location: Chr3:89622329-89660753 bp, + strand Genetic Position: Chr3, 39.19 cM, cytoband F2
• Alliance: Adar^tm1.1Phs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:142338
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A floxed neo selection cassette was placed upstream of exon 7 in the SmaI restriction site and an additional loxP site was inserted downstream of exon 9. Correct targeting was confirmed by genomic PCR. LoxP-flanked (floxed) sequence encoded residues 707?870 of the interferon-inducible form of ADAR1 (GenBankTM/EBI accession number AAK16102), including essential regions for editing activity. (J:87715)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Adar Mutation: 71 strains or lines available

References

• Original: J:142338 Hartner JC, et al., ADAR1 is essential for the maintenance of hematopoiesis and suppression of interferon signaling. Nat Immunol. 2009 Jan;10(1):109-15
• All: 19 reference(s)