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Mcm2tm1(cre/ERT2)Scpr
Targeted Allele Detail
Nomenclature
Symbol: Mcm2tm1(cre/ERT2)Scpr
Name: minichromosome maintenance complex component 2; targeted mutation 1, Steven C Pruitt
MGI ID: MGI:3818719
Synonyms: Mcm2IRES-CreERT2, Mcm2tm1(cre)Scpr
Gene: Mcm2  Location: Chr6:88860456-88875762 bp, - strand  Genetic Position: Chr6, 39.64 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:141493
Parent Cell Line:  W4 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Inducible, Recombinase)
Inducer:    tamoxifen
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsAn IRES element, a cre/ESRI fusion gene. and a PGK-neo selection cassette were inserted into exon 16 3' to the stop codon. A cryptic polyA site 3' of the stop codon was deleted in order to permit cre expression. Cre expression was confirmed by EGFP expression after tamoxifen treatment of mice also carrying the Tg(CAG-Bgeo/GFP)21Lbe transgene. MCM2 expression was reduced to 62% of normal in heterozygotes and 35% in homozygotes. (J:141493)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Mcm2     Summary of all recombinase alleles driven by Mcm2. Comparative matrix view of recombinase activities
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 9 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Mcm2 Mutation:  42 strains or lines available
References
Original:  J:141493 Pruitt SC, et al., Reduced Mcm2 expression results in severe stem/progenitor cell deficiency and cancer. Stem Cells. 2007 Dec;25(12):3121-32
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/30/2021
MGI 6.17
The Jackson Laboratory