Agrp^tm1(cre)Lowl
Targeted Allele Detail

Summary

• Symbol: Agrp^tm1(cre)Lowl
• Name: agouti related neuropeptide; targeted mutation 1, Bradford B Lowell
• MGI ID: MGI:3815085
• Synonyms: Agrp-Cre, Agrp-Ires-cre
• Gene: Agrp Location: Chr8:106293327-106306477 bp, - strand Genetic Position: Chr8, 53.04 cM, cytoband D1-D2
• Alliance: Agrp^tm1(cre)Lowl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:140858
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Duplication, Insertion
• Agrp^tm1(cre)Lowl involves 2 genes/genome features (Hsd11b2, Atp6v0d1) View all
• Agrp^tm1(cre)Lowl expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Agrp (MGI:892013)

• Mutation details: An IRES-cre-frt-kan/neo-frt cassette was inserted in frame in exon 5 after the stop codon via homologous recombination. The kan/neo cassette was subsequently removed via in vivo Flp mediated recombination. Crosses to reporter mice detected Cre activity in AGRP+ neurons. The mutation resulted in a duplication of chromosome 8 genomic sequence at least 53 kb in size that inserted into intron 1 of the nearby Ripor1 (Fam65a) locus. The duplicated sequence includes the entire Hsd11b2 locus, the entire Atp6v0d1 locus and at least 11.7 kb of the Agrp locus. The insertion appears to be in the ~9 kb intron upstream of the translation start site-containing exon 2,however, the effect on Ripor1 expression was not specifically analyzed. (J:140858)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Agrp (mouse) Summary of all recombinase alleles driven by Agrp. Comparative matrix view of recombinase activities Alliance mouse Agrp gene page

Expression

In Mice Carrying this Mutation:

60 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Agrp Mutation: 18 strains or lines available

References

• Original: J:140858 Tong Q, et al., Synaptic release of GABA by AgRP neurons is required for normal regulation of energy balance. Nat Neurosci. 2008 Sep;11(9):998-1000
• All: 174 reference(s)