Get1^tm1Dpc
Targeted Allele Detail

Summary

• Symbol: Get1^tm1Dpc
• Name: guided entry of tail-anchored proteins factor 1; targeted mutation 1, David P Corey
• MGI ID: MGI:3814434
• Synonyms: Get1^tm1.1Tmos, Wrb^fl
• Gene: Get1 Location: Chr16:95946607-95959052 bp, + strand Genetic Position: Chr16, 56.84 cM
• Alliance: Get1^tm1Dpc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:237954
• Parent Cell Line: iTL BA1 (ES Cell)
• Strain of Origin: (C57BL/6NTac x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted 5' to exon 2, and a loxP- and FRT-flanked neomycin cassette was placed 3' to exon 4. For this allele (delta N), the FRT-flanked neomcycin cassette was deleted by crossing the mice with a widely-expressed Flp strain of unknown genetic background, leaving a single residual FRT site and exons 2-4 flanked by loxP sites. (J:237954)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Get1 Mutation: 28 strains or lines available

References

• Original: J:237954 Vogl C, et al., Tryptophan-rich basic protein (WRB) mediates insertion of the tail-anchored protein otoferlin and is required for hair cell exocytosis and hearing. EMBO J. 2016 Dec 01;35(23):2536-2552
• All: 2 reference(s)