Grm7^tm1Betz
Targeted Allele Detail

Summary

• Symbol: Grm7^tm1Betz
• Name: glutamate receptor, metabotropic 7; targeted mutation 1, Heinrich Betz
• MGI ID: MGI:3809761
• Synonyms: mGluR7a^AAA
• Gene: Grm7 Location: Chr6:110622542-111544191 bp, + strand Genetic Position: Chr6, 51.19 cM
• Alliance: Grm7^tm1Betz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:138831
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The last three codons in exon 10 comprising the C-terminal PDZ-ligand motif were replaced with alanine codons via homologous recombination. A floxed neo cassette was also inserted and subsequently removed by Cre mediated recombination in the ES cells. Western blot analysis indicated that protein levels were reduced by about 35% and 15% in the brains of homozygous and heterozygous mice, respectively. (J:138831)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Grm7 Mutation: 124 strains or lines available

References

• Original: J:138831 Zhang CS, et al., Knock-in mice lacking the PDZ-ligand motif of mGluR7a show impaired PKC-dependent autoinhibition of glutamate release, spatial working memory deficits, and increased susceptibility to pentylenetetrazol. J Neurosci. 2008 Aug 20;28(34):8604-14
• All: 1 reference(s)