Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze
Targeted Allele Detail
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Symbol: |
Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 6, Hongkui Zeng |
MGI ID: |
MGI:3809522 |
Synonyms: |
Ai6, R26floxstop-EGFP, R26ZsGreen, ROSA26LoxP-STOP-LoxP-ZsGreen1, Rosa-CAG-LSL-ZsGreen1-WPRE, RosaZsG, ROSAZsGreen, Rs-ZsGreen-R, ZsGrn |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Alliance: |
Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze page
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Allele Type: |
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Targeted (Conditional ready, Reporter) |
Mutation: |
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Insertion
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Mutation details: The Rosa-CAG-LSL-ZsGreen1-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), ZsGreen1 sequence (from pZsGreen1-N1 (Clontech); a human codon optimized/enhanced green fluorescent protein engineered for brighter fluorescence and higher expression in mammalian cells), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus.
(J:155793)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:155793 Madisen L, et al., A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci. 2010 Jan;13(1):133-40 |
All: |
286 reference(s) |
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