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E2f3tm2.1Gle
Targeted Allele Detail
Summary
Symbol: E2f3tm2.1Gle
Name: E2F transcription factor 3; targeted mutation 2.1, Gustavo Leone
MGI ID: MGI:3806814
Synonyms: E2f3a-
Gene: E2f3  Location: Chr13:30090558-30170046 bp, - strand  Genetic Position: Chr13, 13.09 cM, cytoband A3.3
Alliance: E2f3tm2.1Gle page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:138289
Parent Cell Line:  TC1/TC-1 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA floxed neo cassette was inserted 108 bp upstream of the E2f3a start codon and a third loxP site was inserted approximately 300 bp downstream of exon 1a. In vivo cre-mediated recombination removed the neo cassette and exon 1a. Western blot analysis of MEFs confirmed the absence of expression of the E2f3a isoform with retention of expression of the E2f3b isoform. (J:138289)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structure(s)
Tumor Data
List all tumor models in MMHCdb carrying E2f3tm2.1Gle
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any E2f3 Mutation:  32 strains or lines available
References
Original:  J:138289 Tsai SY, et al., Mouse development with a single E2F activator. Nature. 2008 Aug 28;454(7208):1137-41
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/12/2024
MGI 6.23
The Jackson Laboratory