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Shhtm6Amc
Targeted Allele Detail
Nomenclature
Symbol: Shhtm6Amc
Name: sonic hedgehog; targeted mutation 6, Andrew P McMahon
MGI ID: MGI:3775846
Synonyms: Shh::GFP
Gene: Shh  Location: Chr5:28456840-28467101 bp, - strand  Genetic Position: Chr5, 14.39 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:132152
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  Not Specified
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
 
Mutation detailsA GFP cassette was inserted into exon 3 and an frt-flanked neo cassette was inserted between exon 2 and 3. The presence of the fusion protein and the absence of the endogenous product was confirmed by western blot analysis on E9.5 embryo extracts. This allele produces functional albeit inefficient protein product. (J:132152)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Shh Mutation:  22 strains or lines available
References
Original:  J:132152 Chamberlain CE, et al., Notochord-derived Shh concentrates in close association with the apically positioned basal body in neural target cells and forms a dynamic gradient during neural patterning. Development. 2008 Mar;135(6):1097-106
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
01/21/2020
MGI 6.14
The Jackson Laboratory