About   Help   FAQ
Tg(CAG-SAC/EGFP)35Rang
Transgene Detail
Nomenclature
Symbol: Tg(CAG-SAC/EGFP)35Rang
Name: transgene insertion 35, Vivek Rangnekar
MGI ID: MGI:3772442
Synonyms: SAC/35, SAC-GFP line 35, Tg(ACTB-SAC/EGFP)35Rang
Transgene: Tg(CAG-SAC/EGFP)35Rang  Location: unknown  
Transgene
origin
Strain of Origin:  (C57BL/6 x C3H)F1
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
 
Mutation detailsThe pCA/SAC-GFP transgene was designed with a chicken beta-actin promoter (and cytomegalovirus (CMV) enhancer) upstream of a fusion gene containing a Par-4 SAC domain (Pawr gene, amino acids 137-195) cDNA fused to an enhanced green fluorescent protein (EGFP), all followed by a polyA sequence. The resulting transgenic offspring were bred to (C57BL/6 x C3H)F1 mice. Founder line 35 mice (SAC/35; found to have 6-8 copies of the transgene). Widespread expression of the transgene is observed in all tested tissues (with some differential tissue-specific regulation of transgene expression or protein stability reported). EGFP expression level is appropriate for immunoblots, but not sufficient enough for fluorescence of flow cytometry applications. (J:125633)
Phenotypes
Loading...
View phenotypes for all genotypes (concatenated display).
Disease models
Loading...
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:125633 Zhao Y, et al., Cancer resistance in transgenic mice expressing the SAC module of Par-4. Cancer Res. 2007 Oct 1;67(19):9276-85
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
08/25/2015
MGI 6.0
The Jackson Laboratory