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Transgene Detail
Symbol: Tg(CAG-SAC/EGFP)35Rang
Name: transgene insertion 35, Vivek Rangnekar
MGI ID: MGI:3772442
Synonyms: SAC/35, SAC-GFP line 35, Tg(ACTB-SAC/EGFP)35Rang
Transgene: Tg(CAG-SAC/EGFP)35Rang  Location: unknown  
Strain of Origin:  (C57BL/6 x C3H)F1
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
Mutation detailsThe pCA/SAC-GFP transgene was designed with a chicken beta-actin promoter (and cytomegalovirus (CMV) enhancer) upstream of a fusion gene containing a Par-4 SAC domain (Pawr gene, amino acids 137-195) cDNA fused to an enhanced green fluorescent protein (EGFP), all followed by a polyA sequence. The resulting transgenic offspring were bred to (C57BL/6 x C3H)F1 mice. Founder line 35 mice (SAC/35; found to have 6-8 copies of the transgene). Widespread expression of the transgene is observed in all tested tissues (with some differential tissue-specific regulation of transgene expression or protein stability reported). EGFP expression level is appropriate for immunoblots, but not sufficient enough for fluorescence of flow cytometry applications. (J:125633)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Original:  J:125633 Zhao Y, et al., Cancer resistance in transgenic mice expressing the SAC module of Par-4. Cancer Res. 2007 Oct 1;67(19):9276-85
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.16
The Jackson Laboratory