Chrna4^tm3Lst
Targeted Allele Detail

Summary

• Symbol: Chrna4^tm3Lst
• Name: cholinergic receptor, nicotinic, alpha polypeptide 4; targeted mutation 3, Henry Lester
• MGI ID: MGI:3770442
• Synonyms: alpha4YFP
• Gene: Chrna4 Location: Chr2:180664104-180685339 bp, - strand Genetic Position: Chr2, 103.54 cM
• Alliance: Chrna4^tm3Lst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:123687
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A YFP gene with an HA epitope tag at the 5' end was inserted in-frame into exon 5, which encodes the M3-M4 cytoplasmic loop of the encoded protein. This allele expresses a fusion protein under the control of the endogenous gene. In confocal brain images of coronal sections, high levels of immunofluorescence were detectable in the medial habenula, thalamus, substantia nigra pars compacta (SNC), and the interpendicular nucleus. Moderate expression levels were also detected in superior colliculus, cerebral cortex, retrosplenial granular cortex and subiculum. (J:123687)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Chrna4 Mutation: 42 strains or lines available

References

• Original: J:123687 Nashmi R, et al., Chronic nicotine cell specifically upregulates functional alpha4* nicotinic receptors: basis for both tolerance in midbrain and enhanced long-term potentiation in perforant path. J Neurosci. 2007 Aug 1;27(31):8202-18
• All: 8 reference(s)