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Chrna4tm3Lst
Targeted Allele Detail
Nomenclature
Symbol: Chrna4tm3Lst
Name: cholinergic receptor, nicotinic, alpha polypeptide 4; targeted mutation 3, Henry Lester
MGI ID: MGI:3770442
Synonyms: alpha4YFP
Gene: Chrna4  Location: Chr2:181022311-181043579 bp, - strand  Genetic Position: Chr2, 103.54 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:123687
Parent Cell Line:  CJ7 (ES Cell)
Strain of Origin:  129S1/Sv-Oca2+ Tyr+ Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
 
Mutation detailsA YFP gene with an HA epitope tag at the 5' end was inserted in-frame into exon 5, which encodes the M3-M4 cytoplasmic loop of the encoded protein. This allele expresses a fusion protein under the control of the endogenous gene. In confocal brain images of coronal sections, high levels of immunofluorescence were detectable in the medial habenula, thalamus, substantia nigra pars compacta (SNC), and the interpendicular nucleus. Moderate expression levels were also detected in superior colliculus, cerebral cortex, retrosplenial granular cortex and subiculum. (J:123687)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Chrna4 Mutation:  29 strains or lines available
References
Original:  J:123687 Nashmi R, et al., Chronic nicotine cell specifically upregulates functional alpha4* nicotinic receptors: basis for both tolerance in midbrain and enhanced long-term potentiation in perforant path. J Neurosci. 2007 Aug 1;27(31):8202-18
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
07/07/2020
MGI 6.15
The Jackson Laboratory