Chrna4^tm2.1Dra
Targeted Allele Detail

Summary

• Symbol: Chrna4^tm2.1Dra
• Name: cholinergic receptor, nicotinic, alpha polypeptide 4; targeted mutation 2.1, John Drago
• MGI ID: MGI:3767042
• Synonyms: S248F
• Gene: Chrna4 Location: Chr2:180664104-180685339 bp, - strand Genetic Position: Chr2, 103.54 cM
• Alliance: Chrna4^tm2.1Dra page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:125305
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: In vivo Cre-mediated recombination removed the floxed neo cassette leaving just the S248F (TCG to TTT) in exon 5 in the putative M2 region. This mutation had little effect on nicotinic acetylcholine receptor ligand binding; however, dopamine release mediated by receptor complexes containing the mutated subunit was decreased at high agonist concentrations. (J:125305)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Chrna4 Mutation: 42 strains or lines available

References

• Original: J:125305 Teper Y, et al., Nicotine-induced dystonic arousal complex in a mouse line harboring a human autosomal-dominant nocturnal frontal lobe epilepsy mutation. J Neurosci. 2007 Sep 19;27(38):10128-42
• All: 1 reference(s)