Klf2^tm1Mlkn
Targeted Allele Detail

Summary

• Symbol: Klf2^tm1Mlkn
• Name: Kruppel-like transcription factor 2 (lung); targeted mutation 1, Mark L Kahn
• MGI ID: MGI:3765423
• Synonyms: Klf2^fl
• Gene: Klf2 Location: Chr8:73072906-73075498 bp, + strand Genetic Position: Chr8, 34.99 cM, cytoband C1
• Alliance: Klf2^tm1Mlkn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:127228
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of exon 2 and a second loxP site was placed downstream of exon 3. A FRT-flanked Neo resistance cassette was placed further downstream of exon 3. The Neo cassette was excised in vivo using a CMV-Flp transgene, leaving one FRT site behind. Cre mediated recombination will excise exons 2 and 3, which encode 331 of the 354 amino acids of the Klf2 protein, including all three zinc fingers, the nuclear localization sequence, and >90% of the transactivation domain. (J:127228)

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Klf2 Mutation: 12 strains or lines available

References

• Original: J:127228 Lee JS, et al., Klf2 is an essential regulator of vascular hemodynamic forces in vivo. Dev Cell. 2006 Dec;11(6):845-57
• All: 14 reference(s)