Cpa3^tm2Hrr
Targeted Allele Detail

Summary

• Symbol: Cpa3^tm2Hrr
• Name: carboxypeptidase A3, mast cell; targeted mutation 2, Hans-Reimer Rodewald
• MGI ID: MGI:3760646
• Synonyms: Mc-cpa^Y356L,E378A
• Gene: Cpa3 Location: Chr3:20269784-20296345 bp, - strand Genetic Position: Chr3, 6.25 cM
• Alliance: Cpa3^tm2Hrr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:125964
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The endogenous gene has been mutated by homologous recombination to contain two point mutations in exon 11, A1073T and A1139C (per NCBI Entrez mRNA NM_007753), and thus to encode a protein harboring the amino-acid substitutions Tyr356Leu and Glu378Ala, respectively. A single loxP site remains in intron 10. Although western blot analysis of lysates of peritoneal mast cells demonstrates that mast cells from homozygous mutant mice express ~80% as much protein as those from normal mice, the lysates contain no detectable carboxypeptidase activity against the test substrate N-[4-methoxyphenylazoformyl]-Phe-OH. (J:125964)
• Inheritance: Recessive

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cpa3 Mutation: 30 strains or lines available

References

• Original: J:125964 Schneider LA, et al., Molecular mechanism of mast cell mediated innate defense against endothelin and snake venom sarafotoxin. J Exp Med. 2007 Oct 29;204(11):2629-39
• All: 4 reference(s)