About   Help   FAQ
Pdx1tm1Egs
Targeted Allele Detail
Nomenclature
Symbol: Pdx1tm1Egs
Name: pancreatic and duodenal homeobox 1; targeted mutation 1, Edouard G Stanley
MGI ID: MGI:3688420
Synonyms: Pdx1GFP/W
Gene: Pdx1  Location: Chr5:147270097-147275848 bp, + strand  Genetic Position: Chr5, 86.84 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:110137
Parent Cell Line:  W9.5/W95 (ES Cell)
Strain of Origin:  129S1/Sv-Oca2+ Tyr+ Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
 
Mutation detailsA targeting vector inserted GFP coding sequences and an frt-flanked hygromycin resistance gene into exon 1. Transient transfection with a vector encoding flp recombinase removed the hygromycin resistance gene and one of the frt sites from targeted ES cells. The expression pattern of GFP in homozygous mutant embryos and adult mice mirrors that of the endogenous gene. (J:114151)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 10 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pdx1 Mutation:  22 strains or lines available
References
Original:  J:110137 Holland AM, et al., A mouse carrying the green fluorescent protein gene targeted to the Pdx1 locus facilitates the study of pancreas development and function. Genesis. 2006 Jun;44(6):304-7
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
02/04/2020
MGI 6.14
The Jackson Laboratory