Tg(CMV-IL3,CSF2,KITLG)1Eav
Transgene Detail

Summary

• Symbol: Tg(CMV-IL3,CSF2,KITLG)1Eav
• Name: transgene insertion 1, CJ Eaves
• MGI ID: MGI:3653745
• Synonyms: 3/GM/SF, SGM3, Tg(hSCF/hGM-CSF/hIL3
• Transgene: Tg(CMV-IL3,CSF2,KITLG)1Eav Location: unknown
• Alliance: Tg(CMV-IL3,CSF2,KITLG)1Eav page

Transgene origin

• Strain of Origin: (C57BL/6 x C3H/HeN)F1

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(CMV-IL3,CSF2,KITLG)1Eav expresses 3 genes
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CSF2 (1437)

  Csf2	(MGI:1339752)

  human

  IL3 (3562)

  Il3	(MGI:96552)

  human

  KITLG (4254)

  Kitl	(MGI:96974)

• Mutation details: cDNAs encoding human interleukin 3, colony stimulating factor 2 (granulocyte-macrophage) and kit ligand were independently ligated into expression cassettes in which the cDNA resides downstream of the cytomegalovirus (CMV) promoter/enhancer sequence and is followed by human growth factor intronic and polyadenylation sequences. A transgenic mouse that was shown by ELISA to have serum levels of all three growth factors at ng/ml levels was used to establish a line with the same phenotype. (J:87755)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 7 strains available Cell Lines: 0 lines available

References

• Original: J:87755 Nicolini FE, et al., NOD/SCID mice engineered to express human IL-3, GM-CSF and Steel factor constitutively mobilize engrafted human progenitors and compromise human stem cell regeneration. Leukemia. 2004 Feb;18(2):341-7
• All: 36 reference(s)