Trpc6^tm1Lbi
Targeted Allele Detail

Summary

• Symbol: Trpc6^tm1Lbi
• Name: transient receptor potential cation channel, subfamily C, member 6; targeted mutation 1, Lutz Birnbaumer
• MGI ID: MGI:3623135
• Gene: Trpc6 Location: Chr9:8544143-8680742 bp, + strand Genetic Position: Chr9, 2.46 cM, cytoband A1
• Alliance: Trpc6^tm1Lbi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:106082
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaces exon 7, which encodes amino acids 584-670 comprising transmembrane domains 4 and 5 and a small part of the pore domain. Segments of mRNA upstream or downstream of the deletion cannot be amplified by RT-PCR from total lung RNA of homozygous mutant mice. (J:106082)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Trpc6 Mutation: 67 strains or lines available

Notes

The reported phenotypes did not differ among mice from backcross generations N1 through N10 to C57BL/6J.

Trpc3 mRNA is elevated 3- to 5-fold in thoracic aorta and 2-fold in cerebral arteries of mutant mice compared to wild-type littermates, and Trpc7 mRNA is increased 2-fold in mutant cerebral arteries; levels of other Trpc mRNAs do not differ between mutant and control vessels.

References

• Original: J:106082 Dietrich A, et al., Increased vascular smooth muscle contractility in TRPC6-/- mice. Mol Cell Biol. 2005 Aug;25(16):6980-9
• All: 70 reference(s)