Gja1^{tm6.1(Gjb3)Kwi}
Targeted Allele Detail

Summary

• Symbol: Gja1^{tm6.1(Gjb3)Kwi}
• Name: gap junction protein, alpha 1; targeted mutation 6.1, Klaus Willecke
• MGI ID: MGI:3620584
• Synonyms: Cx43³¹
• Gene: Gja1 Location: Chr10:56253297-56266519 bp, + strand Genetic Position: Chr10, 28.64 cM
• Alliance: Gja1^{tm6.1(Gjb3)Kwi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:106509
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: Crossing mice bearing Gja1^tm6(Gjb3)Kwi with mice expressing Cre recombinase in the germline resulted in excision of the coding region of Gja1, placing that of Gja3, which had been inserted between the Gja1 termination codon and its downstream DNA, under control of the Gja1 regulatory sequences. Immunoblot analysis demonstrates the absence of GJA1 protein and the presence of GJA3 protein, at dosage dependent levels, in lysates of heart and brain from mice bearing this allele. (J:106509)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gja1 Mutation: 59 strains or lines available

References

• Original: J:106509 Zheng-Fischhofer Q, et al., Connexin31 cannot functionally replace connexin43 during cardiac morphogenesis in mice. J Cell Sci. 2006 Feb 15;119(Pt 4):693-701
• All: 1 reference(s)