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Targeted Allele Detail
Symbol: Car4tm1Sly
Name: carbonic anhydrase 4; targeted mutation 1, William S Sly
MGI ID: MGI:3611770
Synonyms: CAIVdl1Sws, CA IV KO
Gene: Car4  Location: Chr11:84848612-84856870 bp, + strand  Genetic Position: Chr11, 51.34 cM
Germline Transmission:  Earliest citation of germline transmission: J:103742
Parent Cell Line:  Other (see notes) (ES Cell)
Strain of Origin:  129S1/Sv-Oca2+ Tyr+ KitlSl-J/J
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsThe self-excising tACE-CRE-neo cassette replaced exons 1b, 2, 3, and 9 amino acids of exon 4. This sequence encodes all three active site His residues in the 146 amino acids of the protein that immediately follow the signal sequence. The resulting frameshift produced an early termination in exon 4. Absence of transcript was confirmed by RT-PCR of mutant brain, heart, kidney and muscle. Western blot analyses of the same tissues did not detect protein. (J:103742)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Car4 Mutation:  19 strains or lines available
ES cell line = W9.5s
Original:  J:103742 Shah GN, et al., Carbonic anhydrase IV and XIV knockout mice: roles of the respective carbonic anhydrases in buffering the extracellular space in brain. Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16771-6
All:  9 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.17
The Jackson Laboratory