Rasgrf1^tm4.1Pds
Targeted Allele Detail

Summary

• Symbol: Rasgrf1^tm4.1Pds
• Name: RAS protein-specific guanine nucleotide-releasing factor 1; targeted mutation 4.1, Paul D Soloway
• MGI ID: MGI:3611767
• Synonyms: Flox-R, Rasgrf1^tm4Pds
• Gene: Rasgrf1 Location: Chr9:89791961-89909030 bp, + strand Genetic Position: Chr9, 47.31 cM
• Alliance: Rasgrf1^tm4.1Pds page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:103751
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to flank the direct repeat sequence downstream of the differentially methylated domain (DMD). A neomycin resistance cassette flanked by a FRT sites that was included downstream of the floxed sequence was removed via FLPe expression. (J:117666)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rasgrf1 Mutation: 65 strains or lines available

References

• Original: J:103751 Yoon B, et al., Rasgrf1 imprinting is regulated by a CTCF-dependent methylation-sensitive enhancer blocker. Mol Cell Biol. 2005 Dec;25(24):11184-90
• All: 3 reference(s)