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Nr1i2tm1Sakl
Targeted Allele Detail
Nomenclature
Symbol: Nr1i2tm1Sakl
Name: nuclear receptor subfamily 1, group I, member 2; targeted mutation 1, Steven A Kliewer
MGI ID: MGI:3609633
Synonyms: PXR-, Pxrtm1Glaxo-Wellcome
Gene: Nr1i2  Location: Chr16:38248323-38294824 bp, - strand  Genetic Position: Chr16, 26.64 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:103505
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA neomycin resistance gene replaced the first coding exon, which includes the first zinc finger of the DNA-binding domain, via homologous recombination. Northern blot and RT-PCR detected transcript ~200 bp shorter than the wild-type transcript, as expected. Sequence analysis revealed that exon 1 was fused to exon 3 in the mutant animal. The truncated transcript was present at reduced levels. If this RNA is translated from a spurious initiation site, the protein is expected to be nonfunctional. (J:103505)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 3 strains available      Cell Lines: 0 lines available
Carrying any Nr1i2 Mutation:  21 strains or lines available
References
Original:  J:103505 Staudinger JL, et al., The nuclear receptor PXR is a lithocholic acid sensor that protects against liver toxicity. Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3369-74
All:  26 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
10/10/2017
MGI 6.10
The Jackson Laboratory