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Pik3r1tm1Lca
Targeted Allele Detail
Nomenclature
Symbol: Pik3r1tm1Lca
Name: phosphoinositide-3-kinase regulatory subunit 1; targeted mutation 1, Lewis C Cantley
MGI ID: MGI:3607981
Synonyms: P85alphaloxP, Pik3r1flox, pik3r1loxP
Gene: Pik3r1  Location: Chr13:101680761-101768217 bp, - strand  Genetic Position: Chr13, 53.92 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:102176
Parent Cell Line:  TC1/TC-1 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
 
Mutation detailsA targeting vector was designed such that exon 7 is flanked by loxP sequences. Upon cre-mediated deletion of the exon, splicing of upstream exons 6, 1b or 1c directly into exon 8 results in a frameshift mutation producing an immediate stop codon. As a result the p85alpha isoform is truncated and p55alpha and p50alpha are essentially abolished. Flp mediated recombination removed the neo cassette. Western blot demonstrates the floxed allele is expressed just like the wild-type. (J:102176)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pik3r1 Mutation:  18 strains or lines available
References
Original:  J:102176 Luo J, et al., Class IA phosphoinositide 3-kinase regulates heart size and physiological cardiac hypertrophy. Mol Cell Biol. 2005 Nov;25(21):9491-502
All:  39 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
04/13/2021
MGI 6.16
The Jackson Laboratory