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Ccktm1Dgen
Targeted Allele Detail
Nomenclature
Symbol: Ccktm1Dgen
Name: cholecystokinin; targeted mutation 1, Deltagen
MGI ID: MGI:3604503
Gene: Cck  Location: Chr9:121489824-121495694 bp, - strand  Genetic Position: Chr9, 72.43 cM, cytoband F4
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:101679
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Project Collection: Deltagen
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
 
Mutation detailsA bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. RT-PCR analysis detects a high level of gene transcript in the brain cortex, moderate levels in whole brain, subcortical region, cerebellum and olfactory bulb, and low levels in brainstem, spinal cord, eye, Harderian glands, heart, lung, liver, pancreas, kidney, spleen, thymus, lymph nodes, skin, gallbladder, urinary bladder, pituitary gland, adrenal gland, salivary gland, skeletal muscle, tongue, stomach, small intestine, cecum, colon, testis, epididymis, seminal vesicle, coagulating gland, prostate gland, ovary, uterus and white fat. However, beta-galactosidase expression from this allele is detectable in only in brain, kidney and testis, with moderate lacZ expression detected in some regions of the whole mount brain including areas of the cortex and hippocampus. On coronal sections of brain, very weak staining was observed in a few cells of the pyramidal cell layer in hippocampus. Strong, scattered expression was observed in renal medulla and papilla. Strong staining was also observed in a few spermatogenic cells in the testis.
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 36 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Cck Mutation:  20 strains or lines available
Notes
See also, data as provided by Deltagen, Inc.
References
Original:  J:101679 Deltagen Inc, NIH initiative supporting placement of Deltagen, Inc. mice into public repositories. MGI Direct Data Submission. 2005;
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
09/12/2017
MGI 6.10
The Jackson Laboratory