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Gabrqtm1Dgen
Targeted Allele Detail
Nomenclature
Symbol: Gabrqtm1Dgen
Name: gamma-aminobutyric acid (GABA) A receptor, subunit theta; targeted mutation 1, Deltagen
MGI ID: MGI:3604492
Gene: Gabrq  Location: ChrX:72825178-72842602 bp, + strand  Genetic Position: ChrX, 37.29 cM, cytoband A6
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:101679
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Project Collection: Deltagen
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Disruption caused by insertion of vector
 
Mutation detailsA bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. RT-PCR analysis detects a high level of gene transcript in the subcortical region of the brain, moderate levels in whole brain, cortex, brainstem, olfactory bulb, spinal cord, lung, spleen, pituitary gland, adrenal gland and white fat and low levels in eye, Harderian glands, heart, liver, kidney, thymus, lymph nodes, skin, gallbladder, urinary bladder, stomach, colon, testis, epididymis, seminal vesicle, coagulating gland, prostate gland, ovary and uterus. Beta-galactosidase expression from this allele is detectable in brain, eye, spleen, heart, lung and liver. Most striking staining was observed in spleen with very strong lacZ expression detected in the capsule and trabeculae. Strong X-Gal staining was observed on coronals sections of the brain in the hypothalamus, amygdala and preoptic area. Weak expression was detected in a few cells of the brainstem. Expression was also detected in a small region of thalamus in the wholemount brain. Strong staining was observed in a few cells of the heart and bronchioles of the lung as well as in some blood vessels of the lung and liver. Weak expression was detected in the eye in a few cells of the ciliary body.
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 48 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gabrq Mutation:  3 strains or lines available
Notes
See also, data as provided by Deltagen, Inc.
References
Original:  J:101679 Deltagen Inc, NIH initiative supporting placement of Deltagen, Inc. mice into public repositories. MGI Direct Data Submission. 2005;
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
09/19/2017
MGI 6.10
The Jackson Laboratory