Rr27^tm3Tilg
Targeted Allele Detail

Summary

• Symbol: Rr27^tm3Tilg
• Name: regulatory region 27; targeted mutation 3, Shirley M Tilghman
• MGI ID: MGI:3580481
• Synonyms: H19^{Ku site-}, H19^tm5Tilg
• Gene: Rr27 Location: Chr7:142133885-142136283 bp Genetic Position: Chr7, Syntenic
• Alliance: Rr27^tm3Tilg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:98880
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutation: Insertion
• Mutation details: Mutations to change the ATCGAT to CACGCA were introduced into the DNA binding sites of hypersensitivity sites 1 and 2 of the imprinting control region. A loxP site flanked neomycin resistance gene was excised upon expression of cre recombinase in testes. (J:98880)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr27 Mutation: 7 strains or lines available

References

• Original: J:98880 Katz DJ, et al., Functional characterization of a novel Ku70/80 pause site at the H19/Igf2 imprinting control region. Mol Cell Biol. 2005 May;25(10):3855-63
• All: 1 reference(s)